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Image Search Results
Journal: Scientific Reports
Article Title: TUSC3 Loss Alters the ER Stress Response and Accelerates Prostate Cancer Growth in vivo
doi: 10.1038/srep03739
Figure Lengend Snippet: (a) shTUSC3 and control (scrambled shRNA) PC3 and DU145 cells, respectively, were evaluated using transmission electron microscopy in ultrathin sections. Only cells with intact plasma membrane, nucleus, nucleolus, mitochondria, ER and Golgi apparatus were included into the analysis. We examined 20 cells of each cell type. Arrows indicate branching or dilatation of ER cisternae. Scale bars correspond to 2 μm. TUSC3 silencing promotes prostate cancer xenograft growth. (b) shTUSC3 and control (scrambled shRNA) PC3 and DU145 cells, respectively, were injected into the flanks of 6–8-week old male nude mice. Tumor growth was monitored and is shown as mean tumor size (+/− SEM). (c) Representative tumor formed by the TUSC3 silenced PC3 xenograft in the right flank of a Foxn1 nu/nu mouse (top left). Sustained downregulation of TUSC3 in mouse tumors was analyzed by qRT-PCR (top right) and immunohistochemistry (TUSC3 IHC, middle graphs). Major differences in N-glycosylation in mouse tumors were not identified by Concanavalin A lectin histochemistry (ConA lectin, bottom graphs).
Article Snippet: Lectin histochemistry and cytochemistry was performed using
Techniques: Control, shRNA, Transmission Assay, Electron Microscopy, Clinical Proteomics, Membrane, Injection, Quantitative RT-PCR, Immunohistochemistry, Glycoproteomics